Investigation of serum leptin, ghrelin, irisin, insulin levels and their correlations in cattle with subclinical ketosis

Abstract

In this study, it was aimed to investigate the correlations with leptin, ghrelin, irisin and insulin levels in the blood serum of cattle with subclinical ketosis. For this purpose, 10 healthy and 10 Holstein cattle with subclinical ketosis obtained from farms in Burdur region were used. A diagnosis of subclinical ketosis was made according to the Rothera test performed in milk, by performing a general clinical examination of the animals. Blood samples were taken from vena jugularis into tubes without anticoagulant. Serum leptin, ghrelin, irisin and insulin levels were measured in the obtained sera using commercial ELISA kits. In cows with subclinical ketosis, serum ghrelin, leptin, irisin and insulin values were increased compared to the control group (p