Rapid isolation of rosmarinic acid from Ocimum basilicum using flash chromatography

Abstract

In this study; it was aimed at the isolation of rosmarinic acid (RosA) from Ocimum basilicum (OB) with a selective extraction and rapid isolation technique using flash chromatography (FC). For this purpose, dried leaves of OB were extracted with n-hexane, dichloromethane, and methanol, respectively. The methanol extract (ME) was extracted with dichloromethane and ethyl acetate using separation funnel by suspending with water. The ethyl acetate layer was dried with anhydrous Na2SO4 and the solvent was evaporated to give a rosmarinic acid-rich extract. Then the ethyl acetate extract (EAE) was separated over silica gel column using FC, and thus RosA was isolated with high purity.