Dergiler / Hacettepe Üniversitesi Eczacılık Fakültesi Dergisi / 2022 / Cilt: 42 - Sayı: 3
Experimental Design Optimized Chromatographic Determination of Pseudoephedrine and Cetirizine in Bulk and Tablets
- Sayfa
- 165–174
- DOI
- —
Özet
Chromatographic separation of cetirizine and pseudoephedrine was established using cyanopropyl column and mobile phase composed of aqueous phase (20 mM ammonium acetate buffer, pH 5.5) and acetonitrile (44:56v/v) pumped at 1 mL/minute, analytes were detected at 240 nm. The linearity of the method was between 25- 135 μg/mL and 1.5 –7.5 μg/mL for pseudoephedrine and cetirizine, respectively. Pseudoephedrine and cetirizine detection limits were 5.34 and 0.13 μg/mL, respectively, whereas their quantitation limits were 16.17 μg/mL and 0.40 μg/mL for cetirizine. The method’s recoveries were (99.81% ± 0.60 for pseu- doephedrine and 100.08 ± 0.52 for cetirizine), indicating consistent good and consistent recoveries. Also low relative standard deviations (< 2%) for repeatabil- ity and intermediate precision were obtained supporting method’s high precision. Quantification of pseudoephedrine and cetirizine in bulk and combined tablet formulation was successfully carried out using the validated method.
Abstract
Chromatographic separation of cetirizine and pseudoephedrine was established using cyanopropyl column and mobile phase composed of aqueous phase (20 mM ammonium acetate buffer, pH 5.5) and acetonitrile (44:56v/v) pumped at 1 mL/minute, analytes were detected at 240 nm. The linearity of the method was between 25- 135 μg/mL and 1.5 –7.5 μg/mL for pseudoephedrine and cetirizine, respectively. Pseudoephedrine and cetirizine detection limits were 5.34 and 0.13 μg/mL, respectively, whereas their quantitation limits were 16.17 μg/mL and 0.40 μg/mL for cetirizine. The method’s recoveries were (99.81% ± 0.60 for pseu- doephedrine and 100.08 ± 0.52 for cetirizine), indicating consistent good and consistent recoveries. Also low relative standard deviations (< 2%) for repeatabil- ity and intermediate precision were obtained supporting method’s high precision. Quantification of pseudoephedrine and cetirizine in bulk and combined tablet formulation was successfully carried out using the validated method.