In-vitro cytotoxicity of white vinegar: Effects of different concentrations

Abstract

Aim: We compared the cytotoxic effects of different concentration of white vinegar, that easy to apply, unexpensive and effective on microorganisms. Method: The cells L929 mouse skin fibroblasts were used for the experiments. Cytotoxicity was evaluated colourimetrically by MTT [4,5-dimethylthiazol-2-yl-2,5 diphenyltetrazolium bromide; thiazolyl blue] proliferation assay. Results: White vinegar was found to be moderately cytotoxic at its all concentrations, in the 10-minutes incubation period. While the other concentrations were moderately cytotoxic, only 100% white vinegar concentration was found to be severely cytotoxic, in the 24-hours incubation period. Conclusion: It was observed that white vinegar was moderately cytotoxic on L929 fibroblast at all concentrations, in the 10-minutes incubation period