Determination of antimutagenic properties of Helichrysum plicatum DC. subsp. plicatum by Micronuclei and COMET Methods

Abstract

Helichrysum plicatum DC. subsp. plicatum (Asteraceae) is a medicinal plant and natural product. This plant is commonly found in Anatolia and has been used in folk medicine for thousands of years. There are many studies in the literature investigating the biological properties of this plant, but few studies have investigated anti-mutagenic activities. In this study, the antimutagenic of different concentrations of Helichrysum plicatum DC. subsp. plicatum methanol extract (5, 10, 25, 50, 100, 200 and 300 µgr / mL) was investigated using the micronuclei and the single cell gel electrophoresis test systems. Aflatoxin B1 was used as positive control and pure water was used as negative control. As a result, Helichrysum plicatum DC. subsp. plicatum was found to have antimutagenic activity in both test systems and the most effective results were obtained from 100 ugr / mL concentration.