Secondary Metabolites from Thraustochytrium aureum and Their Biological Activity

Abstract

Phytochemical investigation of Thraustochytrium aureum led to the isolation of eleven compounds including ergosterol (1), 7-dehydroporiferasterol (2), (22E,24R)-ethylcholesta-7,22-dien-3β,5α,6β-triol (3), poriferasterol glucoside (4), perlolyrine (5), pyrrolezanthine-6-methyl ether (6), 3-(3-aminopropyl)-6-[(4-hydroxyphenyl)methyl]-2,5- piperazinedione (7), 5-methyluracil (8), 1H-indole-3-carboxylic acid (9), adenosine (10), and p-hydroxybenzoic acid (11). Screening for α-glucosidase inhibitory, antimicrobial and cytotoxic activities of the extracts and isolated compounds were carried out. The n-hexane extract was the most active, which showed strong α-glucosidase inhibitory activity with IC50 of 48.22 μg/mL, inhibition against E. faecalis, S. aureus and C. albicans microorganism strains with MIC values of 128, 64 and 64 μg/mL, respectively. Compound 6 have shown to be the most active among isolated compounds, which inhibited α-glucosidase with IC50 value of 7.96 μg/mL; E. faecalis and C. albicans microorganism strains with MIC values of 64 and 16 μg/mL and inhibited the growth of A549, HepG2, MCF7 and LNCaP cancer cell lines with IC50 values of 62.26, 41.03, 57.21 and 43.23 μg/mL, respectively.