Th e Eff ect of Diff erent Storage Temperature on Hu Ram Sperm Parameters

Abstract

In order to explore the most suitable temperature for the preservation of Hu ram semen at room temperature, the semen samples were placed at 15°C, 20°C, and 25°C respectively. Th e energy metabolism of enzyme activity and triphosphate adenosine (ATP) was detected in the process of preservation. Diff erent parameters such as functional integrity, survival time, reactive oxygen species (ROS) content, superoxide dismutase (SOD) activity, and energy metabolism of adenosine triphosphate (ATP) were detected. Th e results showed that within 2 days of storage, the sperm progressive motility stored at 25°C was the highest. Within 3~5 days of storage, sperm stored at 20°C have the highest progressive motility. Within 6~9 days of storage, the sperm progressive motility stored at 15°C was signifi cantly higher than that of the other groups (P