The effect of the platelet-rich plasma on osteogenicpotential of the periosteum in an animal bone defect model

Abstract

Objectives: This study aims to investigate whether plasma-richplasma (PRP) enhances the osteogenic potential of periostealgrafts used to repair bone defects and maintains both histologicallyand biomechanically more durable bone tissue. Materials and methods: A standard bone defect was formed tothe left femurs of 54 Sprague-Dawley rats and three groups wereformed. In the first group (n=18), no periosteal repair was donefor bone defect. In the second group (n=18), periosteal graft tissuewas sutured to cover the defect entirely. In the third group (n=18),before periosteal repair, a 1 mL of PRP fibrin was applied into thebone defect. All femoral specimens were compared histologicallyat four and six weeks and biomechanically by three-pointbending test at six weeks after treatment. Results: In the PRP applied group, healing of the bone defectat four weeks was significantly better than the other groups interms of histological new bone formation (p