Lack of association between MMP13 (rs3819089), ADAM12 (rs3740199-rs1871054) and ADAMTS14 (rs4747096) genotypes and advanced-stage knee osteoarthritis

Abstract

Objectives: The aim of this study was to investigate the relationshipbetween MMP13 rs3819089, ADAM12 rs3740199 and rs1871054,and ADAMTS14 rs4747096 genotypes in patients with radiologicallydiagnosed knee osteoarthritis (OA).Patients and methods: A total of 300 patients (68 males, 232 females;mean age: 61.6 years; range, 25 to 89 years) who were admitted to theorthopedics and traumatology clinic and diagnosed with knee OAaccording to the 2000 American College of Rheumatology (ACR)criteria between October 2018 and March 2019 were prospectivelyanalyzed. Patients with Grades III-IV OA according to the KellgrenLawrence (K-L) grading system were included in the patientgroup (n=150) and those without radiological features of kneeOA (K-L Grades I-II) were included in the control group (n=150)voluntarily. The presence of single nucleotide polymorphisms (SNPs)in the targeted genes in both groups was assessed by real-timepolymerase chain reaction in the peripheral blood sample.Results: The most common nucleotides in both the control and patientgroups were CG for rs3740199 and CT for rs1871054 in the ADAM12gene, and the most common nucleotides in alleles were GG forMMP13 rs3819089 and AA for ADAMTS14 rs4747096. No statisticallysignificant relationship was detected between the gene polymorphismsand advanced OA.Conclusion: The study results suggest that ADAM12 rs3740199and rs1871054, MMP13 rs3819089, and ADAMTS14 rs4747096polymorphisms have no relationship with knee OA susceptibility in theTurkish population. However, as this is the first study to investigate therelationship between the SNPs of ADAM12, ADAMTS14, and MMP13genes and the development of OA in the Turkish population, it wouldcontribute to our understanding of the molecular bases of OA.