The Role of Platelet Rich Plasma for Preventing Postoperative Peritoneal Adhesions in Adhesive Intestinal Obstruction in Rats

Abstract

Background: Various methods have been investigated to prevent postoperative peritoneal adhesions (PPA). It is thought that wound healing process would be better with the use of platelet rich plasma (PRP). Materials and Methods: The aim of this study was to investigate the role of PRP in the formation of PPA and in preventing adhesion formation in rats. It was also planned to demonstrate the relationship between serum interleukin (IL)-6 and TNF-alpha levels and the severity of adhesions before creating the adhesion model and during sacrification. Rats were divided into 3 groups, each consisting of twelve rats. Groups were classified as; PRP, sham and control group. The rats were sacrified on the 14th day and relaparotomies were performed. Results: The results were evaluated macroscopically and microscopically according to predetermined classifications. When the group treated with PRP was compared with the control groups according to the previously described macroscopic (Nair) and microscopic (Zuhlke) classifications, there was no significant difference in PPA formation both microscopically and macroscopically. It is thought that TNF-alpha and IL-6 should be used as markers in PPA formation process. Conclusion: In the experimentally created adhesion model, the effect of PRP on preventing PPA formation could not be proved