The relationship between the effects of the cold pretreatments of buds on abscisic acid content of anthers and in vitro androgenesis of peppers (Capsicum annuum L.)

Abstract

Buds of Capsicum annuum L. cultivar Malatya were treated cold at +4°C for periods of 48 and 96 hours. The anthers were cultured in the Murashige and Skoog (MS) medium with 4 mg $l^{-1}$ Naphthalene acetic acid (NAA) and 1 mg $l^{-1}$ Benzyladenine (BA), 0.25% activated charcoal. The Abscisic acid (ABA) analyses were performed in anthers taken from the buds that were seperated from the plant (control) and in their cold treated samples at +4°C for 48 and 96 hours and in either their samples that incubated 50 days. The ABA amount of the anthers that had cold treated were lower than the control. The embryo formation was obtained from the control anthers not subjected to cold pretreatments (12.5%).