Purification and Characterization of Van Lake fish (Chalcalburnus tarichii P.1811) liver and brain acethylcholinesterase

Abstract

In this study, acethylcholinesterase was purified from liver and brain of Van Lake fish (Chalcalburnus Tarichii P.1811) by using affinity chromatography technique. The purification folds were determined as 142 and 344 for brain and liver, respectively. The purity of enzyme was controlled with sodium dodecylsulfate-polyacrylamide gel electrophoresis (SDSPAGE). The optimal pH was determined as 7.5-8.5 for brain and liver, respectively. On the other hand, the optimal temperature was determined as 35oC for both liver and brain. Also, the highest activities were observed in concentration of 0.2 M (NH4)2SO4 as ionic strength for both liver and brain.