Çiğ süt, pastörize süt ve beyaz peynir örneklerinde bulunan beta-laktamaz pozitif Staphylococcus'lar üzerine bir araştırma

Investigation about beta-lactamase positive Staphylococcus found in samples of raw milk, pasteurized milk and white cheese

Özet

Çeşitli süt işletmelerinden sağlanan çiğ süt, pastörize süt ve beyaz peynir örneklerinden 73 Staphylococcus izole edilmiştir. Bu bak¬terilerin 59'u (%80.82) S. aureus, 7'si (%9.59) S. epidermis ve 7'si (%9.59) de S.saprophyticus'dur. Bu bakterilerin lodometrik Test ve Kromo-jenik Sefalosporin Test yöntemleriyle beta-laktamaz enzim aktiviteleri tespit edilmiştir. Bu yöntemlere ilave olarak kontrol amacıyla penisilin-G antibiyotiği ile test edilmişlerdir. 73 Staphylococcusp'un 48'i (%65,75) beta-laktamaz pozitif, 25'i (%34,25) beta-laktamaz negatiftir. 48 betalaktamaz pozitif Staphylococcus'ların 46'sı S.aureus 2'si de S. epidermidir. 46 beta-laktamaz pozitif S. aureus/un 8'i (%17.39) çiğ sütten, 7'si (%15.22) pastörize sütten ve 31'i (%67,39) beyaz peynir örneklerinden izole edilmiştir. 2 betalaktamaz pozitif S. epidermidis/in 1'i (%50) çiğ sütten, 1'i (%50) beyaz peynirden izole edilmiştir. 48 beta-laktamaz pozitif Staphylococcus klavulanat-amoksisilin, amikasin, sefazolin, gentamisin, klindamisin ve ofloksasin antibiyotikleriyle test edilmişlerdir. 46 beta-laktamaz pozitif S.aureus'yın 43'ü (%93) klindamisin'e en yüksek oranda dirençli bulunurken 23 (%50) S. aureus de klavulanat-amoksisilin'e dirençli tespit edilmiştir. Beta-laktamaz pozitif 2 (%100) S. epidermidis klindamisin'e, gentamisin'e ve sefazolin'e en yüksek oranda dirençli bulunmuştur. Ofloksasin ve klavulanat-amoksisilin'e %50 oranında dirençli bulunmuşlardır.

Abstract

73 Staphylococcus were isolated from raw milk, pasteurized milk and white cheese samples provided from several milk establishment. Of them; 59 were characterized as Slaureus (80.82%), 7 were S.epidermidis (9.59%) and 7 were S.saprophyticus (9.59 %). The beta-lactamase enzyme activity of these bacteria were also tested by iodometric test and chromogenic cephalosporin test medhods. In addition to these methods; with the aim of control it was tested with penicillin G. ob. Among 73 Staphylococcus; 48 were (65,75%) were beta-lactamase positive, 25 were (34,25%) found as betalactamase negative. Of 48 positive beta-lactamase Staphylococcus; only 2 were characterized as S.epidermidis while 46 were, S. aureus. The 8 (17,39%) of 46 beta-lactamase positive S. aureus were isolated from raw milk, while 7(15,22%) of 46 were isolated from pasteurized milk and 31(67,39%) were from white cheese samples. The one of 2 beta-lactamase was isolated from raw milk while the other one was isolated from white cheese. 48 beta-lactamase positive Staphylococcus were tested with the antibiotics of amoxicillin-clavulanic acid, amikacin, gentamicin, clindamycin and ofloxacin. While, 43 (93%) of 46 beta-lactamase positive S.aureus were found to show most resistance against clindamycin; 23(50%) S.aureus was also found to be resistant against amoxicillin-clavulanic acid. 2 (100%) S. epidermidis which were beta-lactamase positive were also found to be resistant with a ratio of 50% against ofloxacin and amoxicillin-clavulanic acid.