The Effects of Rutaecarpine on Metastatic Prostate Cancer Cells

Abstract

Prostate cancer with an increased incidence in the world is one of the public health-threatening malignancy. Metastatic prostate cancer is an important cause of death in men despite of the combined use of more than one chemotherapeutic drug as well as radiotherapy and supportive treatments. Therefore, there is a need to de velop novel treatment strategies in metastatic prostate cancer patients. The aim of this study was to investigate the potential therapeutic effects of Rutaecarpine (RUT) on metastatic prostate cancer cells. RUT induced cytotoxicity and apoptotic cell death were evaluated by WST-1, Annexin V, AO staining and ELISA assays in PC-3 human metastatic prostate cancer cell line.The viability percentage of PC-3 cells after exposing to different concentrations of RUT treatment significantly decreased in a time and dose dependent manner and the most effective concentrations of RUT was determined as 20 and 40 μM for 48 hours (p