Polistemia Vera Olgularında Thiol-Disülfid Homeostasis

Şule Mine BAKANAY, Sema AKINCI, Fuad MUSTAFAYEV, Senem MARAL, Özcan EREL, Murat ALIŞIK, Aysun ŞENTÜRK YIKILMAZ, İmdat DİLEK

Özet

Giriş: Bu çalışmanın amacı, Polistemia Vera (PV) olgularında; hücre siklusunun çeşitli basamaklarında, apoptozis ve hücre proliferasyonunda önemli rol oynadığı bilinen tiyol-disülfid homeostasisini incelemeyi amaçladık. Yöntemler: Kırkiki PV olgusu 43 sağlıklı kontrol grubuyla karşılaştırıldı. Serum total (–SH + –S–S–) ve nativ (-SH) tiyol seviyeleri her iki grupta ölçüldü. Dinamik disülfid bağlarının yüzdesi ve (–S–S–) and (–S–S–) × 100/(–SH), (–S–S–) × 100/(–SH + –S–S–), ve –SH ×100/(–SH + –S–S–) oranları Erel ve arkadaşlarının yeni metodu ile hesaplandı. PV olguları ve sağlıklı kontrol grubunun verileri kıyaslandı. Sonuçlar: Her iki grubun yaş ve cinsiyet dağılımları benzerdi. PV grubunda nativ tiyol, total tiyol ve nativ/total tiyol seviyeleri istatiksel olarak anlamlı artmıştı. Tartışma: PV hastalığının doğasıyla uyumlu olarak, PV olgularında dengenin proliferasyon yönüne kaydığı gözlemlendi. Total tiyol (–SH + –S–S), nativ tiyol (–SH)ve nativ /total tiyol oranındaki artışı PV hastalığındaki kontrolsüz proliferasyonla açıklayabiliriz. PV olgularında tiyol/disülfid dengesinin indirgeyici tiyol tarafında olduğunu gösterdik. Tüm bu değişiklikler PV hastalığının patofizyolojisinde olan kontrolsüz proliferasyonla ilişkili olabilir.

Abstract

Background: Thiol-disulphide homeostasis has vital role in cell signalling mechanisms, regulation of transcription factors andenzymatic activities, signal transduction and regulation of proliferation rate, apoptosis and detoxification and antioxidantprotective mechanism.Objective: This study aims to demonstrate in Polycythemia Vera (PV) patients the thioldisulphide homeostasis which is known toplay a role in cell proliferation, apoptosis and various steps of cell cycle.Design: Descriptive prospective cross-sectional study.Settings: Yildirim Beyazıt University Hospital Ankara, Turkey between 2016-2018.Method: Forty-two PV patients and 43 healthy controls were included in the study. Serum total (–SH + –S–S–) and native (–SH)thiol levels were measured in all subjects. The amount of dynamic disulphide bonds and, the ratio of (–S–S–) and (–S–S–) × 100/(–SH), (–S–S–) × 100/(–SH + –S–S–), and –SH ×100/(–SH + –S–S–) were calculated with automatic method. The data obtained fromthe patient group were compared with the control group.Main outcome measures: The amount of dynamic disulphide bonds and, the ratio of (–S–S–) and (–S–S–) × 100/(–SH), (–S–S–) ×100/(–SH + –S–S–), and –SH ×100/(–SH + –S–S–) were calculated with automatic method in PV patieint and healthy control group.Results: Both groups were similar in terms of age and gender distribution. Compared with the control group, PV group hadsignificantly higher native thiol, total thiol and nativ/total thiol levels.Limitation: The generalizability of the study's findings were limited by the small sample size.Conclusions: In accordance with the nature of the disease, thiol balance in PV patients was in favor of proliferation. Increased totalthiol (–SH + –S–S), native thiol (–SH) levels and native thiol/total thiol ratio might be associated with uncontrolled proliferation.The balance of the thiol-disulphide homeostasis shifted to reductive thiol side in the PV. This change can provocate proliferationstatus of the disease and/or may be secondary to the disease.Conflict of Interest: No conflict of interest was declared by the authors.