Hepatitis A seroprevalence in patients who admitted to a training and research hospital in Southeast Anatolia

Özet

Objective: In this study; patients admitted to Diyarbakır Gazi Yaşargil Training and Research Hospital were evaluated retrospectively according to age groups for hepatitis A virus seroprevalence.Methods: Anti HAV IgG and Anti HAV IgM test results of patients who admitted to our hospital for various reasons between January 2010 and December 2014 were evaluated. HAV IgG and HAV IgM antibodies were studied via chemiluminescence immune method in Advia Centaur XP (Siemens, Germany) system following the manufacturer's instructions.Results: 97.30% of the samples was positive for Anti HAV IgG and 0.87 of the samples was positive for Anti HAV IgM. Anti HAV IgM positivity was 10% in the 0-10 years age group and 3.09% in the 11-20 age group. We detected advancing age has been found to reduce the incidence of disease and most of the patients encountered disease in childhood and gained immunity.Conclusion: As a result, in this study we found the HAV seroprevalence in the largest hospital of our province. We thought we could contribute; preventive measures, immunization program studies and determination of vaccine efficacy studies with the light of this data.Key words: Hepatitis A Virus, seroprevalence, age groups

Abstract

Objective: In this study, it was aimed to compare the sensitivity of diagnostic tests in patients with a preliminary diagnosis of brucellosis.Methods: We have compared the serological methods, standard tube agglutination test (STA), Coombs Test (CT), Rose Bengal (RBT), and the gel centrifugation test. In patients with a preliminary diagnosis of brucellosis, subjects with a positive test result of RBT has been included in the research and other diagnostic tests STA, CT and Coombs Gel centrifugation tests were performed within the range of same titration.Results: Total 132 patient’s serums were studied. In RBT positive 92 patients’ serums, negative test results were found in 11 with STA, in 9 with CT and in 6 with gel test. While 35 patients were identified to be positive by using Brucella gel test at 1/5120 titer, no positive test results were seen with STA and CT at the same titer. Generally, CT results were one titration below the gel centrifugation test results.Conclusion: In conclusion, RBT and STA were not always adequate to determine the diagnosis of brucellosis. Low titer STA results should be supported by tests such as CT or gel centrifugation and the seroconversion must be monitored. Due to giving fast results, gel centrifugation test can be preferred in diagnosis of Brucellosis.Key words: Brucellosis, gel centrifugation, Coombs test