Effect of Nebivolol on Isolated Rat Bladder Strips Precontracted with Carbachol or Potassium Chloride

Abstract

Objective: One of the proposed mechanism mediating the vasorelaxant effect of nebivolol is based on its agonistic activity on beta-2 and/or beta-3 adrenergic receptors. These receptors are also involved in the relaxation of urinary bladder. The aim of this study was to explore the ability of nebivolol to induce relaxation of the isolated rat bladder strip precontracted with cholinergic stimuli using carbachol or non-cholinergic stimuli using potassium chloride (KCl). Methods: The isolated bladder strips were mounted in organ bath and contracted by KCl (40 μM) or carbachol (1mM) before the cumulative addition of nebivolol (0.0001-100 μM). To investigate the role of beta-adrenergic receptors in the nebivolol-induced relaxant response, some bladder strips were incubated with propranolol (1 μM) for 30 min. Statistical significance was tested by Student’s t-test. p