Dergiler / Celal Bayar Üniversitesi Fen Bilimleri Dergisi / 2018 / Cilt: 14 - Sayı: 2

Evaluation of the Genetic Fidelity of in vitro Raised Plants of Origanum majorana L. Using Random Amplified Polymorphic DNA

Evaluation of the Genetic Fidelity of in vitro Raised Plants of Origanum majorana L. Using Random Amplified Polymorphic DNA

Burcu ÇETİN

Sayfa
237–239
DOI
—

Abstract

Determination of the genetic characters of the plantsobtained using plant tissue culture methods is important. In this study, the geneticfidelity of the plants, which are obtained by the micropropagation of the shoottip explants of the Origanum majoranaL. plant of medicinal and economical value in Murashige and Skoog (MS) mediumwhich contains 1.0 mg/L Benzil Amino Purin (BAP), 0.1 mg/L naphthaleneaceticacid (NAA), 3% sucrose and 0.7% agar, has been investigated by usingrandom amplified polymorphic DNA (RAPD-PCR) technique. Monomorphic bands were obtainedas a result of all of the RAPD-PCR analyses performed. According to the results obtained, no polymorphism was detected among themicropropagated plants.