Study of competitive protein-drug binding by capillary electrophoresis

Abstract

The competitive affinity of two drugs (the anticoagulant warfarin and the diuretic furosemide) to bovine serum albumin (BSA) is studied by capillary electrophoresis. The competition of these two drugs for the same site on BSA is shown. The binding constant between the displacer drug (furosemide) and BSA is estimated relative to the known warfarin-BSA binding constant.