Spectroscopic analyses on the binding interaction of thiosemicarbazone-derivated Cu(II) complex with DNA/BSA

Abstract

Cu(II) complex of 2-hydroxy-5-methoxyacetophenone-N(4)-ethyl thiosemicarbazone (Cu(HMAET)Cl) was synthesized and characterized by spectroscopic methods such as FT-IR, 1H-NMR and UV-Vis. The DNA-binding capacity of the complex was studied with the UV-Vis absorption titrations and ethidium bromide (EB) displacement experiments using E. coli DNA. The results demonstrated that the complex could intercalate in the DNA's base pairs. The binding constant was found as 3.6×108 M−1. Also, the interaction study of the complex with bovine serum albumin (BSA) was investigated using the UV-Vis absorption and fluorescence spectroscopy techniques and it was concluded that the complex interacts strongly with BSA.