Dergiler / Tarla Bitkileri Merkez Araştırma Enstitüsü Dergisi / 2019 / Cilt: 28 - Sayı: 2
Qualitative and Quantitative Determination of Tryptanthrin, Indirubin, Indican and Isatin Indole Alkaloids During Vegetative and Flowering Stages in the Roots and Leaves of Isatis constricta P. H. Davis
- Sayfa
- 59–66
- DOI
- —
Özet
Isatis genus more known as the indigo plant is a plant belonging to the Brassicaceae family in Turkey.Turkey has 31 types and 15 subtypes in various provinces.18 of these species and 3 of the subspeciesare assumed as endemic. All taxa of the genus are extensively used in dyeing and treatment of variousinfections. They contain large number of secondary compounds like phenolics, flavonoids and alkaloids inroot, leaf, flower and fruit parts. Among these indole alkaloids like triptanthrin, indirubin, indican and isatinare used in treatment of many diseases. These are obtained from the leaves and roots are pharmacologicallyactive compounds. This study revealed that this biannual herbaceous plant grows 30 - 50 cm in lengthon volcanic slopes, at 1200 - 1300 meters above sea level. It generally grows in Eastern Anatolia andthe Mediterranean region of Turkey. The study aimed to determine optimum ontogenetic stage of Isatisconstricta to extract natural bio compounds like indirubin, isatin, indican, tryptanthrin. The leaf and rootsamples used in this study were collected from Maden county of Elazığ province. They were tested forthe presence of these compounds during vegetative and flowering period of the plant. The HPLC (High-Performance Liquid Chromatography) analysis results showed that tryptanthrin 0.482 (±0.0072) μg/g andindirubin 0.029 (±0.00007) μg/g has the highest concentrations in the leaf samples obtained during thevegetative period. However, isatin 4.358 (± 0.0985) μg/g and indican 21.330 (± 0.114) μg/g were foundto have the highest concentration in leaf samples obtained during flowering period. When both resultsare compared, it can be seen that the amount of tryptanthrin, isatin, indirubin and indican alkaloids werefound in the leaves of I. constricta higher than to the roots. This research idicate that the most appropriatedevelopment stage of I. constricta to obtain triptanthrin indirubin, indican and isatin compounds.
Abstract
Türkiye’de daha çok çivit otu olarak bilinen İsatis cinsi Brassicaceae familyasına ait bir bitkidir. Türkiye’nin çeşitli yerlerinde bu bitkiye ait 31 tür ve 15 alt türü bulunmaktadır. Bu türlerin 18 tanesi ve alttürlerin 3 tanesi endemiktir. Cinsin bütün taksonları çeşitli enfeksiyonların tedavisinde ve boyamada yaygın olarak kullanılmaktadır. Kök, yaprak, çiçek ve meyve kısımlarında fenolikler, flavonoidler ve alkaloidler gibi çok sayıda ikincil bileşik bulunmaktadır. Bunlar arasında triptanthrin, indirubin, indican ve isatin gibi alkaloidler birçok hastalığın tedavisinde kullanılmaktadır. Bunlar hem yapraklar hem de köklerden elde edilen farmakolojik olarak aktif bileşiklerdir. Bu çalışmada kullanılan bitkisi 30 - 50 cm kadar boylanan, deniz seviyesinden 1200 - 1300 metre yüksekte volkanik yamaçlarda bulunan iki yıllık önemli otsu bir bitkidir. Bu bitki genellikle Doğu Anadolu ve Türkiye’nin Akdeniz bölgesinde doğal olarak yetişmektedir. Bu çalışmanın