Astragalus diphtherites FENZL var. diphtherites ve Astragalus gymnalopecias RECH. FIL’in Gövde ve Kök Kısımlarından Farklı Çözücüler ile Elde Edilen Özütlerin İnvitro Antioksidan ve Antimikrobiyal Özelliklerinin Belirlenmesi

Determination of in vitro antioxidant and antimicrobial properties of shoot and root extracts of Astragalus diphtherites FENZL var. diphtherites and Astragalus gymnalopecias RECH. FIL. obtained by different solvents.

Özet

Bu çalışmanın amacı farkı polariteye sahip çözücü serilerindengeçiren Astragalus diphtherites var. diphtherites (A. diphtherites)ve Astragalus gymnalopecias (A. gymnalopecias) türlerinin gövde vekök kısımlarının antioksidan ve antimikrobiyal özelliklerininaraştırılmasıdır. Antioksidan özellikleri belirlemek için özütlerintotal fenolik, total flavonoid, DPPH, metal şelatlama, indirgemegücü ve hidroksi radikalini söndürme aktiviteleri test edildi. Enyüksek total fenolik bileşen miktarı A. diphtherites’ de gövdedemetanol, kökte ise aseton özütünden elde edilirken A.gymnalopecias’ ta gövdede metanol kökte ise etil asetat özütündetespit edildi. A. diphtherites ve A. gymnalopecias’ ta en yüksek totalflavonoid miktarları ve indirgeme gücü aktiviteleri gövdede asetonkökte ise etil asetat özütlerinden elde edildi. A. diphtherites için enyüksek DPPH radikalini söndürme aktivitesi, gövdede metanolkökte ise aseton özütünde, A. gymnalopecias’ ta gövdede metanolkökte ise etil asetat özütünde tespit edildi. A. diphtherites’ te gövdekısmından elde edilen metanol ve kök kısmından elde edilen hekzanözütleri, A. gymnalopecias’ ta ise gövde ve kök kısımlarının metanolözütleri en yüksek metal şelatlama aktivitesi gösterdi. Her iki türdede gövde ve kök etil asetat özütleri en yüksek hidroksil radikalisöndürme aktivitesi gösterdi. A. gymnalopecias’ın gövde aseton vemetanol özütlerinin Streptococcus pyogenes’in büyümesi üzerindeinhibisyon etkisine sahip olduğu tespit edildi.

Abstract

Objective of this study was to investigate the antioxidant and antimicrobial properties of shoot and root parts of Astragalus diphtherites var. diphtherites (A. diphtherites) and Astragalus gymnalopecias (A. gymnalopecias) species which were subjected to solvent series with different polarity. Total phenolic, total flavonoid, 2.2-diphenyl-1-picryl-hydrazyl (DPPH), metal chelating, reducing power and hydroxy radicals scavenging activities of the extracts were tested to determine antioxidant properties. The highest total phenolic content of A. diphtherites, was obtained from the methanol extract of shoots and the acetone extract of root parts. On the other hand, the highest total phenolic content in A. gymnalopecias were achieved from methanol extract of shoot parts and the ethyl acetate extract of the root part. The highest amount of total flavonoids and reducing power activities of A. diphtherites and A. gymnalopecias were obtained from the acetone extracts in the shoot part and from the ethyl acetate extracts in the root part. While the highest DPPH radical scavenging activity was determined in the methanol extract in the shoot part and the acetone extract in the root part of A. diphtherites. The highest DPPH radical scavenging activity for A. gymnalopecias was determined in the methanol extract of the shoot part and the ethyl acetate extract of the root part. The highest metal chelating activity was seen in the methanol extracts from shoot parts and in the hexane extracts from the root part of A. diphtherites. The ethyl acetate extracts of the shoot and root part in both species showed the highest hydroxyl radical scavenging activity. It was determined that acetone and methanol extracts of the shoot part of A. gymnalopecias have inhibition effect on the growth of Streptococcus pyogenes.