Pomegranate (Punica granatum L.) Peel Extract: Potential Cytotoxic Agent against Different Cancer Cell Lines

Özet

The aim of the present study was to investigate effects of pomegranate peel (PP) extract on differenthuman cancer cell lines. MTT was performed to estimate cytotoxic effects of PP extract against HTB140,HTB177, MCF7, HCT116 human cancer cell lines and MRC-5 normal fibroblasts. Clonogenic assay was usedto reveal cell survival after the treatment with PP extract. Cell cycle analysis was done using flow cytometry.Wound healing assay was applied to estimate inhibitory effects of PP extract on migration of cancer cells. Theresults showed that PP extract expressed selective cytotoxicity for cancer cells compared to normal cell line.Analyzed cancer cell lines displayed individual variations in sensitivity to PP extract reflected through changesin clonogenic survival, cell cycle distribution and migration, which may be due to the specific nature of eachtested cell line. In conclusion, PP extract exhibits good inhibitory effects on tested cancer cell lines.