Di-, and Triterpenoids Isolation and LC-MS Analysis of Salvia marashica Extracts with Bioactivity Studies

Özet

In this study, dichloromethane, acetone, and methanol extracts of the aerial parts of the Salviamarashica plant which is an endemic species to Anatolia, were investigated. The total phenolic amounts of theseextracts were determined as pyrocatechol equivalent and total flavonoids as quercetin equivalent. Antioxidantactivity was determined by four complementary methods including inhibition of lipid peroxidation (by βcarotene color expression), DPPH free radical scavenging activity, ABTS cation radical scavenging activity andCUPRAC methods. Anticholinesterase activity of the extracts was investigated by the Ellman method againstacetylcholinesterase (AChE) and butyrylcholinesterase (BChE) enzymes. Viability and cytotoxic activity testswere carried out on the fibroblast L929 cells and cytotoxic A549 lung cancer cells, respectively. Thetriterpenoids and diterpenoids constitute the major secondary metabolites of the S. marashica acetone andmethanol extracts isolated by chromatographic methods. Their structures were determined based onspectroscopic methods, namely NMR and mass analyses. Ten terpenoids were obtained from either acetone ormethanol extracts of the S. marashica. Seven of them were triterpenoids, elucidated as lupeol, lupeol-3-acetate,lup-12, 20(29)-diene, lup-20(29)-ene, α-amyrin-tetracosanoate, oleanolic acid and ursolic acid besides a steroidβ-sitosterol. Two abietane diterpenes, abieta-8,11,13-triene (1) and 18-acetoxymethylene-abieta-8,11,13-triene(2), were obtained from the acetone extract which were isolated from a Salvia species for the first time in thepresent study. The methanol extract was found to be very rich in rosmarinic acid determined by LC-MS/MSanalysis.