Anti-Angiogenic Effect of Matrine in in-Vivo ROP-Mouse- Model

Özet

Purpose: To evaluate the effect of intravitreal (IV) Matrine (Mat) injection on retinal endothelial cell proliferation (REP), retinal morphology,and apoptotic activity in hyperoxia induced (HIR) in-Vivo ROP- mouse model using C57BL/6J.Methods: Total of 48 C57BL/6J mice were divided in 6 group each including 8. Group A and B-S exposed to room air were negative controlgroups; group C, D-S, E and F exposed to 75% ± 2% oxygen from postnatal day (PD) 7 to PD 12 comprised HIR groups. Group C and D-S werecontrol groups, group E and F were low and high dose IV-Mat injected groups. Group A and C received no IV injection, B-S and D-S received 1μL IV sterile 0.9% NaCl injection, E and F received 0.78μg and 6.25μg IV-Mat injection respectively. After enucleation, we measured REP bycounting neovascular tufts in cross-sections and examined histological, ultrastructural changes via light and transmission electron microscope.Apoptosis was detected using terminal deoxynucleotidyl transferase-mediated nick end-labeling.Results: No REP was detected in negative control groups. REP levels were signifi cantly different between low- and high-dose Mat groups.REP levels were signifi cantly lower in the Mat groups, compared to the control groups. The incidence of mitochondrial dysmorphology andapoptosis were lower in groups receiving Mat compared to the control groups.Conclusion: Mat seems to suppress REP and has anti-apoptotic activity in the HIR mouse model.