Antibacterial and Radical Scavenging Activities of Ruta buxbaumii Poir. (Rutaceae) Growing in Raman Mountain-Batman

Özet

The members of the Rutaceae genus have found a wide application area in traditional medicinein many countries from ancient times to today; they have been used for many purposes inTurkey. Ruta species have many pharmacological properties such as inducing abortion,antirheumatic, hypoglycemic, anthelmintic, antipyretic, antiepileptic, antidiabetic, againstepilepsy, vertigo, headache and in eye diseases, anthelmintic and against poisoning. In thispaper, it was aimed to determine the antimicrobial and radical scavenging activities of methanol(MeOH), petroleum ether (PE), ethyl acetate (EtOAc) and ethanol (EtOH) extracts of Rutabuxbaumii Poir. growing naturally in Raman mountain, Batman. Antimicrobial activities ofextracts were evaluated using disc diffusion method. Two Gram-positive bacteria(Staphylococcus aureus ATCC 25923, Streptococcus pyogenes ATCC 19615) and two Gramnegative bacteria (Pseudomonas aeruginosa ATCC 27853, Escherichia coli ATCC 25922)were used to determine the antibacterial activity. Results demonstrated that although R.buxbaumii extracts showed close antibacterial effects, EtOAc extract showed the highest effectagainst S. pyogenes with 23.5±1.2 inhibition zones, while EtOH extract showed the lowesteffect against P. aeuroginosa with 11.8±0.2 inhibition zones. All the extracts showed noclearance of zone inhibition for against E. coli. Antioxidant properties and activities wereevaluated by using total phenolic content (TPC), total flavonoid content (TFC), DPPH (2,2-diphenyl-1-picryl-hydrazyl-hydrate) free radical scavenging activity. The results showed thatwhile activity of the EtOH extract of R. buxbaumii was the highest (54.10±0.13 mg GAE/gextract) for total phenolic substance content, MeOH and EtOH extracts were found to be higher(47.52±0.19 mg QE/g extract and 46.86±0.16 mg QE/g extract) for total flavonoid content,respectively. Also, radical scavenging activities such as (DPPH) of extracts were investigated,and it was revealed that EtOH extracts (73.6 %), MeOH (60 %), EtOAc (40 %) and PE extract(25 %), respectively. From these results indicates that this species can be used in thepharmaceutical application as a valuable bioproduct with new functional properties in foods.