Foeniculum vulgare miller (Rezene) meyvesi uçucu yağının lethal doz 50 (LD50) düzeyi ve sağlıklı ve diyabetli farelerde hipoglisemik etkisinin araştırılması

Investigation of the level of the lethal dose 50 (LD50) and the hypoglycemic effect in healthy and diabetic mice of foeniculum vulgare mill. fruit essential oil extract

Özet

Amaç: Foeniculum vulgare Mill, (rezene) meyvesi uçucu yağının, lethal doz 50 düzeyinin ve sağlıklı ve diyabetli farelerde hipoglisemik etkisinin araştırılması. Yöntem: Sağlıklı ve alloksanla diyabet oluşturulmuş farelere Foeniculum vulgare Mill, uçucu yağı ekstresi, glibenclamide (referans) ve serum fizyolojik (kontrol) oral yolla verilip; sıfırıncı, birinci, ikinci, dördüncü ve 24. saatlerde, farelerin kuyruk veninden kan alınarak açlık kan şekeri düzeyleri ölçüldü; sonuçlar tek yönlü varyans analizi ile test edildi. Bulgular: Foeniculum vulgare Mill, ekstresinin; alloksanla diyabet oluşturulmuş farelerde serum fizyolojik grubuna göre açlık kan şekerini yalnızca 4. satte (p 0.05); glibenclamidein, serum fizyolojik grubuna göre açlık kan şekerini birinci (p 0.05). Sağlıklı farelerde gruplar arasında açlık kan şekeri yönünden yapılan ölçümlerde anlamlı bir fark elde edilmedi. Sonuç: Foeniculum vulgare Mill, meyvesi uçucu yağ ekstresinin, referans ilaç olarak kullanılan glibenclamide ile aynı düzeyde olmak üzere, sadece dördüncü saatte hipoglisemik etki gösterdiği gözlendi. Birinci, ikinci ve 24. saatlerde ise Foeniculum vulgare Mili. ekstresi hipoglisemik etki göstermedi. Foeniculum vulgare Mill. Uçucu yağ ekstresinin LD50 dozu ise 6.149 ml/kg olarak tespit edildi.

Abstract

Aim: Investigation of The Level of The Lethal Dose 50 and the hypoglycemic effect of Foeniculum vulgare Mill, fruit essential oil extract in healthy and diabetic mice. Method: Extract of Foeniculum vulgare Mill., glibenclamide (as a reference group) and physiologic saline (control group) were administrated per or ally to the healthy and diabetic mice inducede with alloxan. Before treatment in the first, second, third, fourth and 24th hours, blood was taken from the vena coccygea of mice. Blood glucose levels were measured. Results were tested by One-way ANOVA. Results: When compared with physiologic saline group, it was observed that Foeniculum vulgare Mill, extract decreased blood glucose values significantly in a diabetic mice inducede with alloxan in the fourth (p<0.05), this effect was not observed in the first, second and 24th hour. Glibenclamide decreased blood glucose values significantly in the first (p<0.05), second, fourth and 24"' hours (p<0.01). When compared with Foeniculum vulgare Mill., it was observed that glibenclamide decreased blood glucose values more significantly in the first (p<0.001), second (p<0.05) and 24th hours (p<0.01), there was not a significant difference between two groups in the fourth hour (p>0.05). There was not a significant difference of blood glucose values measurements among groups of healthy mice. Conclusion: It was observed that the essential oil extract of Foeniculum vulgare Mill, has significantly hypoglycemic effect%in the only fourth hour when compared with glibenclamide which was used as a reference agent. It was not showed that hypoglycemic effect of the extract of Foeniculum vulgare Mill, in the first, second or 24th hours. The lethal dose 50 of the extract of Foeniculum vulgare Mill was defermined as 6.149 ml/kg..

Anahtar kelimeler: Fare,Modeller, teorik,Modeller, hayvan,Foeniculum,Öldürücü doz 50,Yağlar, uçucu,Diabetes mellitus