Phytochemical and Biological Investigations on Cephalaria anatolica

Abstract

The aerial parts of Cephalaria anatolica yielded two previously undescribed hederagenin-type triterpene glycosides, namely cephosides B (1) and C (2), in addition to eleven known natural compounds. The new compounds were identified as 3-O-β-D-glucopyranosyl-(1  3)--D-glucopyranosyl-(1  3)-α-Lrhamnopyranosyl-(1 glucopyranosyl ester (1) and 3-O-α-L-rhamnopyranosyl-(1  4)--D-glucopyranosyl-( 1  3)-α-Lrhamnopyranosyl-(1 glucopyranosyl ester (2) by 1D and 2D NMR spectroscopic data, HRESIMS analysis and chemical evidence. The two new triterpenoid saponins and the n-butanol extract were evaluated for their hemolytic activity in human erythrocyte cells and immunomodulatory properties in activated whole blood cells by PMA plus ionomycin.While only n-butanol extract increased IL-1β concentration strongly with a value of 760.52 pg/mL, cephoside C and n-butanol extract augmented a slight IFN-γ secretion with values of 4874.11 and 4750.20 pg/mL, respectively. None of the compounds changed IL-2 levels significantly and showed hemolytic activity