New 5-oxomilbemycins from a Genetically Engineered Strain Streptomyces bingchenggensis BCJ60

Abstract

Two new 5-oxomilbemycins, 27-aldehyde-5-oxomilbemycin β12 (1) and 2-hydroxymilbemycin K (2), were isolated from a genetically engineered strain Streptomyces bingchenggensis BCJ60. Their structures were determined by comprehensive analyses of its 1H and 13C NMR, COSY, HMQC, and HMBC spectroscopic, HRESI-MS mass spectrometric and comparison with data from the literature. Preliminary studies showed that 27aldehyde-5-oxomilbemycin β12 (1) and 2-hydroxymilbemycin K (2) possessed strong acaricidal and nematocidal activities