Comparative Effects of Flavonoids from Fructus Sophoraeon Rat Osteoblasts in vitro

Özet

Fructus Sophorae(FS), the dry mature fruit of SophorajaponicaLeguminosae, is a valuabletraditional Chinesemedicine resource with flavonoids as the major active ingredients. To identify the plant-derived estrogen-like flavonoids serving as potential osteoporosis chemopreventive agents, we isolated and identified 27 flavonoids compounds,including 17compounds obtainedfor the first time fromFS. To screen out the flavonoids with estrogen-like biological behavior from the 11 high yield compounds, we set up an in vitroscreening system in rat osteoblast MC3T3-E1 cellsincluding MTTassay, alkaline phosphatase staining and Alizarin red S staining assayto examine the effects on cell proliferation, differentiation and mineralization of osteoblastcells respectively. Six flavonoids, including genistein, sophoricoside, sophorabioside, sophoraflavonoloside, nicotiflorin and rutin,significantly increased the cellular activity of MC3T3-E1 cells. Furthermore, blocking the estrogen receptor signaling by tamoxifencompromised the effects above significantly, suggestingthe 6 compounds behave as estrogen-like reagents. Moreover, the interaction between thesix flavonoids from FSand estrogen receptorwas clarified by molecular dockingmethod from Glide XP. Collectively, being used as food and medicine in China, FS is rich in flavonoids with estrogen-like effects, may be used as healthy supplementary in treating postmenopausal women osteoporosis.