Antibacterial Efficacy of Some Antiseptics and Disinfectants against Common Bacterial Agents Isolated from Horses in Turkey

Özet

Nowadays, many disinfectants and antiseptics are used fordecontamination purposes in equine hospitals, on racetracks,and breeding farms, but generally, these antimicrobial agentsare not tested against commonly encountered pathogens,and they are used with unknown antimicrobial efficacy. Theantimicrobial efficacies of ethanol, chlorhexidine, povidoneiodine, sodium hypochloride, peroxymonosulfate compound,and benzalkonium chloride were analyzed using the quantitative suspension test method against the field isolates ofEscherichia coli, Pseudomonas aeruginosa, Salmonella spp.,Streprococcus zooepidemicus, Streptococcus equi, Rhodococcusequi, and Staphylococcus auerus, which are the most frequently encountered pathogens of equines, in the presence oforganic load (10% fetal bovine serum) after 1 min, 5 mins, and30 mins contact times at 20°C. A log reduction of five or more(5 log ≤) in cfu counts of the tested pathogens was consideredas effective for each disinfectant and antiseptic. According tothe results, except for sodium hypochloride in the 1/100 dilution, all other disinfectants and antiseptics achieved a minimum 5 log reduction and were found to be effective againstall tested isolates. Decreased dilutions and/or direct use of thesodium hypochloride should be tested against the same bacterial agents, as well as with multiple field strains. In addition,reference strains of the microorganisms should be evaluatedin further studies.