The efficacy of dietary savory essential oil on reducing the toxicity of aflatoxin B1 in broiler chicks

Broyler piliçlerde rasyona katılan geyikotu esansiyel yağının aflatoksin B1 toksisitesinin azaltılmasındaki etkinliği

Özet

Bu çalışma, 42 gün süreyle beslenen broyler piliçlerde yeme katılan geyioktu esansiyel yağının, aflatoksin B1 (AFB1)’in büyüme performansı, serum biyokimyası ve hümoral immün yanıtlar üzerine olan zararlı etkilerini yoketme kapasitesinin değerlendirilmesi amacıyla yapıldı. Randomize tasarım ile 22 faktöryel düzenlemede, 300 günlük broyler piliçler 15 kanatlı içeren beş tekrarlı dört tedavi grubu olacak şekilde ayarlandı. Piliçler 7. güne kadar temel rasyon ve daha sonra deneysel diyet ile beslendi. Besinsel tedaviler 500 mg kuru madde olarak geyioktu/kg içeren veya içermeyen 0 ve 0.5 mg AFB1/kg’dan oluştu. Rasyona aflatoksin eklenmesi ağırlık kazancını ve yem tüketimini azaltırken (P 0.05) belirlendi. Geyioktu ilavesinin aflatoksinin büyüme performansı üzerine olumsuz etkilerini azalttığı ve aflatoksine maruz kalan broylerlerde serum biyokimyası ve hümoral immun yanıt üzerine hafif bir olumlu etki sağladığı sonucuna varıldı.

Abstract

The aim of this study was to evaluate the capability of savory essential oil in counteracting the deleterious effects of aflatoxin B1 (AFB1) on growth performance, serum biochemistry, and humoral immune responses in broiler chickens fed 42 days of age. At a 22 factorial arrangement in completely randomized design, 300 day-old broiler chicks were assigned to four treatments with five replicates of 15 birds. Chickens were fed the basal diet up to day 7 of age and then fed the experimental diets. The dietary treatments involved of 0 and 0.5 mg of AFB1/kg with or without 500 mg of savory/kg dry matter. The addition of aflatoxin to diet decreased (P<0.05) the weight gain and feed intake and resulted in a poor feed conversion ratio. Birds in the AFB1 group had lower level of albumin, but higher levels of creatinine and liver enzymes in the serum as compared with the control group. The addition of savory to the contaminated diet diminished (P<0.05) the inhibitory effects of dietary AFB1 on the growth performance and the level of liver enzymes in serum. The addition of aflatoxin to diet caused a decrease and savory supplementation caused an increase in antibody titer against the Newcastle virus, and interaction among the factors was differ (P<0.05). The main effects and interaction on antibody titer against influenza virus were not differ (P>0.05). The addition of savory reduced the adverse effects of aflatoxin on growth performance and provided slight positive effect on serum biochemistry and humoral immune responses in broilers exposed aflatoxin.