Dergiler / Kafkas Üniversitesi Veteriner Fakültesi Dergisi / 2013 / Cilt: 19 - Sayı: 4
The impacts of prebiotic supplementation on humoral and cell-mediated immune responses of broilers under combined stresses caused by feed restriction and Salmonella enteritidis challenge
- Sayfa
- 641–645
- DOI
- —
Özet
Bu çalışma, prebiyotik ilavesinin yem kısıtlaması Salmonella enteritidis inokulasyonu ile kombine stres altındaki broylerlerde sıvısal ve hücresel immun yanıt üzerine etkileri araştırmak üzere gerçekleştirildi. Rastgele tam blok tasarım içinde 23 faktoriyel düzenlemede 320 erkek boyler (Ross 308) 4 tekrar olmak üzere 8 tedavi grubuna bölündü. Bu faktörlerin ve onların etkileşiminin hücresel immun cevap üzerin etkilerini belirlemek üzere kutanöz bazofil aşırı duyarlılık testi (CBH), Newcastle hastalığı (NDV) virüsü ve koyun eritrositlerine (SRBC) karşı gelişen antikor titreleri ve lenfoid organların göreceli ağırlıkları belirlendi. Hücresel immun yanıtı prebiyotik 15. günde arttırırken yem kısıtlamasını 30.günde azalttığı ve faktörler arası etkileşimin faklı (P 0.05).
Abstract
This experiment was conducted to investigate the impacts of prebiotic on humoral and cell-mediated immune responses of broilers under combined stresses caused by feed restriction and Salmonella enteritidis challenge. 320 male broilers (Ross 308) at a 23 factorial arrangement in randomized complete block design were divided into 8 treatment groups with 4 replicates per each. The Impact of these factors and their interaction on cell-mediated immune response to cutaneous basophil hypersensivity (CBH) test, antibody titer response against Newcastle disease virus (NDV) and sheep red blood cell (SRBC), and the relative weight of lymphoid organs were measured. Prebiotic caused an increase and feed restriction caused a decrease in cell-mediated immune responses on days 15 and 30 respectively, and interaction among the factors was differ (P<0.05). Also, factors interaction had a significant effect on antibody titer response against NDV (P<0.05) at day 27. Salmonella and feed restriction significantly reduced the relative weight of thymus at day 21 and bursa of Fabricius at day 42, respectively (P<0.05). Differences among other measured parameters were not significant (P>0.05).