In Vitro Evaluation Of Naproxen Metabolite, O-Desmethylnaproxen On A Mouse Connective Tissue Fibroblast Cells

Abstract

The aim of this in vitro study was assessment of the cytotoxic level of 6-O-desmethylnaproxen. A mouse connective tissue fibroblast cell line, L929 was exposed to naproxen and 6-O-desmethylnaproxen in different concentrations for 24- and 48- hours. Cell viability was tested by MTT, while apoptosis was determined by AO/PI double staining method. The control group was free from any agent (DMEM only) and accepted as 100% cell viability. The IC50 results indicated that the inhibition of 50% cell viability was resulted by the highest concentration of 6-O-desmethylnaproxen (0.7 µg/mL) while none of naproxen concentration was caused 50% cell dead. Consequently, to avoid the unacceptable side effects of naproxen metabolites that evacuated by urine; further studies should be conducted to determine the accumulation of naproxen metabolites.