PREPARATION OF FILM ELECTRODE USING DOPANT FOR CONSTRUCTION OF BIOSENSORS SENSITIVE TO HYDROGEN PEROXIDE

Abstract

In this study, a new film electrode for determination of hydrogen peroxide was developed. Polypyrrole-poly (sodium-4-styrenesulphonate) film have been prepared on the platinum electrode by the electropolymerization of pyrrole was carried out in the presence of poly(sodium-4-styrenesulphonate). Optimum conditions of polypyrrole-poly(sodium-4-styrenesulphonate) film was founded. Oxidation of the hydrogen peroxide was examined at a different potential. Therefore, four different potentials (0.4, 0.5, 0.6, and 0.7 V) were determined. Also, concentration of pyrrole and poly(sodium-4-styrenesulphonate) were examined. The effect of the number of cycles in their sensitivity to hydrogen peroxide is investigated. Morphology of electrodes was qualified by means of SEM. Free enzyme work was performed using the prepared film electrode and glucose oxidase. Linear increases were observed in the amperometric response currents of the electrode versus increasing glucose concentrations. It was shown that this film can be used for hydrogen peroxide producing enzymes.