A New Laccase-Based Biosensor for Epinephrine Determination

Abstract

In this paper, a novel amperometric epinephrine (adrenaline) biosensor with immobilization of laccase on polypyrrole–polyvinylsulphonate (PPy-PVS) film has been a complished. Laccase enzyme were immobilized on PPy-PVS film by cross-linking with glutaraldehyde. Determination of EP was carried out by the reduction of enzymatically produced epinephrinequinone at - 0.220 V vs. Ag/AgCl. The effects of pH and temperature were investigated. There are two linear parts in the region between 0.1 µM-1.0 µM and 1.0 µM-10.0 µM. The storage stability and operation stability of the enzyme electrode were also studied. Interference effects were investigated on response of the biosensor.