Thiol – Disulphide Homeostasis in Polycythemia Vera

Aysun ŞENTÜRK YIKILMAZ,Şule Mine BAKANAY,Sema AKINCI,Senem MARAl,Fuad MUSTAFAYEV,Murat ALIŞIK,Özcan EREL,İmdat DİLEK

Özet

Background: Thiol-disulphide homeostasis has vital role in cell signalling mechanisms, regulation of transcription factors and enzymatic activities, signal transduction and regulation of proliferation rate, apoptosis and detoxification and antioxidant protective mechanism. Objective: This study aims to demonstrate in Polycythemia Vera (PV) patients the thioldisulphide homeostasis which is known to play a role in cell proliferation, apoptosis and various steps of cell cycle. Design: Descriptive prospective cross-sectional study. Settings: Yildirim Beyazıt University Hospital Ankara, Turkey between 2016-2018. Method: Forty-two PV patients and 43 healthy controls were included in the study. Serum total (–SH + –S–S–) and native (–SH) thiol levels were measured in all subjects. The amount of dynamic disulphide bonds and, the ratio of (–S–S–) and (–S–S–) × 100/(– SH), (–S–S–) × 100/(–SH + –S–S–), and –SH ×100/(–SH + –S–S–) were calculated with automatic method. The data obtained from the patient group were compared with the control group. Main outcome measures: The amount of dynamic disulphide bonds and, the ratio of (–S–S–) and (–S–S–) × 100/(–SH), (–S–S–) × 100/(–SH + –S–S–), and –SH ×100/(–SH + –S–S–) were calculated with automatic method in PV patieint and healthy control group. Results: Both groups were similar in terms of age and gender distribution. Compared with the control group, PV group had significantly higher native thiol, total thiol and nativ/total thiol levels. Limitation: The generalizability of the study's findings were limited by the small sample size. Conclusions: In accordance with the nature of the disease, thiol balance in PV patients was in favor of proliferation. Increased total thiol (–SH + –S–S), native thiol (–SH) levels and native thiol/total thiol ratio might be associated with uncontrolled proliferation. The balance of the thiol-disulphide homeostasis shifted to reductive thiol side in the PV. This change can provocate proliferation status of the disease and/or may be secondary to the disease. Conflict of Interest: No conflict of interest was declared by the authors.

Abstract

Giriş: Bu çalışmanın amacı, Polistemia Vera (PV) olgularında; hücre siklusunun çeşitli basamaklarında, apoptozis ve hücre proliferasyonunda önemli rol oynadığı bilinen tiyol-disülfid homeostasisini incelemeyi amaçladık. Yöntemler: Kırkiki PV olgusu 43 sağlıklı kontrol grubuyla karşılaştırıldı. Serum total (–SH + –S–S–) ve nativ (-SH) tiyol seviyeleri her iki grupta ölçüldü. Dinamik disülfid bağlarının yüzdesi ve (–S–S–) and (–S–S–) × 100/(–SH), (–S–S–) × 100/(–SH + –S–S–), ve –SH ×100/(–SH + –S–S–) oranları Erel ve arkadaşlarının yeni metodu ile hesaplandı. PV olguları ve sağlıklı kontrol grubunun verileri kıyaslandı. Sonuçlar: Her iki grubun yaş ve cinsiyet dağılımları benzerdi. PV grubunda nativ tiyol, total tiyol ve nativ/total tiyol seviyeleri istatiksel olarak anlamlı artmıştı. Tartışma: PV hastalığının doğasıyla uyumlu olarak, PV olgularında dengenin proliferasyon yönüne kaydığı gözlemlendi. Total tiyol (–SH + –S–S), nativ tiyol (–SH)ve nativ /total tiyol oranındaki artışı PV hastalığındaki kontrolsüz proliferasyonla açıklayabiliriz. PV olgularında tiyol/disülfid dengesinin indirgeyici tiyol tarafında olduğunu gösterdik. Tüm bu değişiklikler PV hastalığının patofizyolojisinde olan kontrolsüz proliferasyonla ilişkili olabilir.