Oxidative Stress Manifestation for Community-Acqiured Pneumonia in Children Living in Endemic Area of Iodine Deficiency

Özet

Objective: We aimed to evaluate the state of oxidative stress, level of molecular dam- age in relationship with the subcellular distribution of iodine in patients with com- munity-acquired pneumonia from the iodine deficiency region. Materials and Methods: Our study involved 70 children, 4-14 years old, with CAP (moderate severity in 35 children (Group 1); severe - in 35 (Group 2)) and 35 healthy children of the control group (Group 3). The state of organificated/inorganic, antiox- idant defense system, stress-dependent systems, apoptosis and the level of molecular damage were assessed in serum samples. Results: Lower concentration of organificated iodine (12% and 59%, respectively) and high concentration of inorganic iodine (21% and 48%, respectively) were found in Groups 1 and 2. Patients of Group 2 had an inverse relationship between the lev- els of iodine distribution for the organic and inorganic iodine (r = - 0.645, p<0.05). In patients of Group 1, this relationship tends to decrease. The oxidative stress indica- tors correlated with disease severity. ROS generation indices in patients of Group 1 is 28.1 (24.2; 32.1) RFU/mg; (p < 0.05); Group 2 – 40.2 (34.2; 42.5) RFU/mg (p < 0.05); Caspase-3 is 25.77 (21.45–32.16) pmol/mg Group 1 and 39.42 (32.41–44.21) pmol/mg Group 2, (respectively, p < 0.05). We found that children with a severe pneumonia have a prooxidant activation, manifested by a significant increase of oxidation-modi- fied proteins [2.21 (1.88–2.53) nmol/mg)] and DNA fragmentation [13.42 (10.3–15.46) %], (p < 0.05). The extent of DNA damage correlates with the extent of apoptosis. Conclusions: Increase in the concentration of inorganic iodine with a simultane- ous decrease in the level of organic iodine in the blood, which leads to a more severe course of pneumonia. Fluctuations in the indices of oxidative stress, the level of mo- lecular damage and the intensity of apoptosis in children with pneumonia correlate with the severity of the disease.