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This study investigated the effect of computer attitude, ownership and use on the computer literacy of science teachers in Nigeria. One hundred and twenty (120) science teachers drawn from the four political divisions of Ogun State, Nigeria were used for the study. Two valid and reliable instruments namely Computer Attitude, Ownership and Use Scale (CAOUS) and Computer Literacy Self Assessment Scale (CLSAS) were used to collect the needed data. Percentages, standard deviation and multiple regression statistics were employed for data analyses. The findings revealed that the science teachers have positive attitude towards computer. Also, a little above half of the science teachers had personal computers and not all the teachers used computers frequently. Finally, computer attitude, ownership and frequency of use jointly predicted the science teachers' computer literacy with the influence of computer ownership being the highest when considered individually. Suggestions based on these outcomes were presented in this paper.